Influência do treinamento físico e da restrição hídrica no estresse oxidativo em ratos

Abstract

The oxygen species (ROS) can act on most cellular components, causing them to change their functions and structures. Its formation occurs during the process of aerobic ATP production, specifically in the chain of mitochondrial electron transport. To prevent the toxic action of RL on cells, healthy individuals have antioxidant defense mechanisms. The imbalance between these defense mechanisms and the RL, is the "oxidative stress". It is known that exercise is a known form of stress, since there is an increase in RL by increasing oxygen consumption and chronic exposure to it, called physical training, is able to trigger adjustments in response to increased production of ROS or that is, chronic exercise results in the formation of oxidants and oxidative stress, and perhaps as a consequence, induces antioxidant enzymes and antioxidant synthesis to minimize the effects of oxidant. The importance of hydration is to consider the need for it especially during physical exercises and thus believes it is appropriate to investigate oxidative stress in thosecircumstances. Objective: To quantify the peroxide content of myocardial membranes of trained rats were not submitted to water restriction. Materials e Methods: 72 rats were divided randomly into six groups; GAS (n = 12) - sedentary group with water ad libitum; GAE (n = 12) - a group trained with water ad libitum; GRHS (n = 12) - sedentary group with water restriction; GRHE (n = 12) - a group trained with water restriction; GAEI (n = 12) - the sedentary with ad libitum water and only one training session - immediately; GRHEI (n = 12) - sedentary group with restriction water and only one training session, immediately. GAS groups served as controls and GAE, and the amount of water consumed by them the basis for the calculation of 25% water restriction GRHS and GRHE respectively. The training program was performed on a treadmill for small animals and the training sessions were held three times per week, lasting 60 minutes, for twelve weeks. To obtain these samples, the animals were sacrificed by decapitation, taking place soon after the surgery. Six animals of GAS, GAE, GRHE and GRHS were sacrificed one hour after the last training session and six to 72 hours thereafter. The groups immediately GAE end GRHE immediately had six of his animals sacrificed one hour after a single session is 72 years and six after the same session. The heart was removed and the fragments of cardiac muscle were frozen for biochemical analysis. Lipoperóxidos de membrane formation methods evaluated by chemiluminescence (CL) stimulated by-butyl hydroperoxide have analyzed by two-way ANOVA Acid Reactive Substances Tilbarbitúrico (TBARS) and total antioxidant capacity (TRAP), compared by student t test for unpaired samples, with p value <0.05 was considered significant. Results: The analysis of the curves in QL shown lipoperoxide membrane levels increased for the trained group and subjected to water restriction in relation to controls. They also show recovery after 72 hours. Data from the TRAP showed a significant consumption of antioxidants is not soluble in trained animals submitted to water restriction. Conclusion: The low-intensity physical training associated with fluid restriction, practiced in the acute or chronic causes increased oxidative stress, although with recovery.